PCR: paths to sensitivity
Careful strategizing helps make PCR sensitive enough for the most challenging experiments.
Nanopores sense protein modifications
Protein nanopores can detect post-translational modifications in proteins.
At last, a method to measure long-range vibrations in proteins may provide clues to the functional relevance of these motions.
Objective comparison of particle tracking methods
The first community competition designed to objectively compare the performance of particle tracking algorithms provides valuable practical information for both users and developers.
Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship
A recently described red-shifted channelrhodopsin permits control of complex behaviors in freely moving adult flies and reveals the functional modulation of courtship behavior by social experience.
Similarity network fusion for aggregating data types on a genomic scale
Similarity network fusion (SNF) is an approach to integrate multiple data types on the basis of similarity between biological samples rather than individual measurements. The authors demonstrate SNF by constructing patient networks to identify disease subtypes with differential survival profiles.
Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT
Variations on point accumulation for imaging in nanoscale topography (PAINT) for super-resolution imaging extend the technology to allow simple imaging of cellular proteins as well as synthetic DNA nanostructures and provide a high level of multiplexing.
Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells
A streamlined, robust sample-preparation method for mass spectrometry–based proteome analysis is reported. All sample preparation steps are carried out in a single enclosed reactor, reducing the potential for contamination and losses, and enabling comprehensive proteome coverage.
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Nature Methods provides techniques for life scientists and chemists. The publication is dedicated to publishing novel methods together with significant advances to tried and tested techniques allowing researchers to perform faster, better and more efficient experimental research.