Nature Methods Magazine April 2014 Issue

Nature Methods magazine 04/2014 cover - free subscription. The April issue of Nature Methods will take a special look at systems studies of microbial cells, cryo-scanning transmission electron tomography, imaging by mas cytometry, culture methods for leukemia stem cells and more. Other articles cover latest news on techniques for life scientists and chemists.

FEATURES

Next-gen immunohistochemistry
The combination of mass spectroscopy with immunohistochemistry allows highly multiplexed, directly quantitative imaging of tissue samples for both basic and clinical research.

Sleep-spindle detection: crowdsourcing and evaluating performance of experts, non-experts and automated methods
A comparative analysis of methods for scoring human sleep data, in particular sleep spindles, from encephalographic recordings is reported. The authors develop methods for crowdsourcing the identification of sleep spindles and compare the detection performance of experts, non-experts and automated algorithms.

Cryo-scanning transmission electron tomography of vitrified cells
Cryo-scanning transmission electron tomography (CSTET) of unstained, fully hydrated vitrified biological specimens is shown to have advantages over cryo-electron tomography (CET), notably at high sample tilts providing greater depth resolution for thick samples.

Dynamic characterization of growth and gene expression using high-throughput automated flow cytometry
An automated flow cytometry setup is described for dynamic and quantitative measurements of yeast growth and molecular phenotypes at high throughput.

Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension
Single-molecule structural transitions involving DNA twisting can be measured with substantially greater spatiotemporal resolution than previously possible with a gold rotor bead tracking (AuRBT) method. This approach uses magnetic tweezers and evanescent darkfield microscopy to track a gold nanoparticle probe attached to a DNA molecule.

CRISPR snapshots of a gene-editing tool
From single-molecule functional studies to atomic-resolution structures, a windfall of data sheds light on the Cas9 mechanism of targeted DNA scission.

Making protein crystals fly
A new device for injecting membrane-protein microcrystals into the path of an X-ray free-electron laser beam improves the efficiency of serial femtosecond crystallography.

FISHing for faster findings
Quick-hybridizing probes help scientists image the high-speed events leading up to gene transcription.

Scoring all human mutations
Combining 63 annotations provides a unified score for the potential deleteriousness of every possible human mutation.

Single cells make the tissue
By pushing throughput, single-cell transcript profiling can replace marker-based sorting and bulk RNA sequencing to redefine tissues from the bottom up.

Interspecies systems biology (a.k.a. interspecies genetics)
The power of genetics in the worm, and in the bacteria that worms eat, is harnessed for a systems view of the effect of diet on a host organism.

Cancer genomes: discerning drivers from passengers
Tumors impart hints at what drives their progression. Parsing those signals takes old and new approaches.

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About Nature Methods Magazine:
Nature Methods provides techniques for life scientists and chemists. The publication is dedicated to publishing novel methods together with significant advances to tried and tested techniques allowing researchers to perform faster, better and more efficient experimental research.

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